Protocols for Bio-optical Measurements
on the R/V Mirai

Overall Sampling Strategy (Please read first)

1. Photosynthesis-Irradiance experiments (PI's)
2. Samples collected for HPLC pigment determinations
3. Measurements of Turner fluorometry
   • Program for calculation of Turner Chlorophyll
4. Measurements of phytoplankton absorption
   • Creating a data CD-ROM
5. Measurements of Coloured Dissolved Organic Matter (CDOM)
6. Samples collected for flow cytometry (picoplankton)
7. Measurements with the SIMBAD radiometer
   • MarRefFile
   • SIMBAD log sheet
   • Example of a sun file
   • Example of a dark file
   • Example of a sea file
   • Calculation of noon in GMT
8. Measurements with the SIMBADA radiometer
   • SIMBADA log sheet
9. Measurements with the hand-held hyperspectral radiometer
10. Continuous measurements with the PAR sensor

Partnership for Observation of the Global Oceans	International Ocean-Colour Coordinating Group